東京大学先端科学技術研究センター/大学院工学系研究科 応用化学専攻/石北研究室
准教授

【若手研究者紹介:034】応用化学専攻 石北研究室 斉藤圭亮 准教授

略歴
2003年 3月 | 筑波大学第三学群工学基礎学類卒業 |
2008年 3月 | 筑波大学大学院数理物質科学研究科博士課程修了 |
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2008年 4月 | 大阪市立大学大学院理学研究科 博士研究員 |
2011年 1月 | 京都大学生命科学系キャリアパス形成ユニット 特定研究員 |
2012年10月 | 科学技術振興機構「藻類バイオエネルギー」領域 さきがけ研究者 |
2013年10月 | 大阪大学大学院理学研究科 助教 |
2014年 6月 | 東京大学大学院工学系研究科 助教 |
2014年10月 | 東京大学先端科学技術研究センター 助教 |
2015年 5月 | 東京大学先端科学技術研究センター 講師 |
2018年11月 | 東京大学先端科学技術研究センター 准教授 |
PUBLICATION
- Keisuke Saito, Koishiro Mukai, and Hitoshi Sumi*,
Chem. Phys. Lett. 401 (2005) 122-129. doi: 10.1016/j.cplett.2004.11.027
“Excited states of pigments in photosystem II reaction centers of photosynthesis: characterization into a central dimer and remaining monomers”
Journal - Keisuke Saito, Tomoaki Kikuchi, Masaru Nakayama, Koishiro Mukai, and Hitoshi Sumi*
J. Photochem. Photobiol. A 178 (2006) 271-270. doi: 10.1016/j.jphotochem.2005.10.038
“A single chlorophyll in each of the core antennas CP43 and CP47 transferring excitation energies to the reaction center in photosystem II of photosynthesis”
Journal - Keisuke Saito, Koishiro Mukai, and Hitoshi Sumi*
Chem. Phys. 326 (2006) 221-229. doi: 10.1016/j.chemphys.2005.12.012
“The importance of a hot-sequential mechanism in triplet-state formation by charge recombination in reaction centers of bacterial photosynthesis”
Journal - Keisuke Saito*, Tomoaki Kikuchi, Koishiro Mukai, and Hitoshi. Sumi,
Phys. Chem. Chem. Phys. 11 (2009) 5290-5301. doi: 10.1039/b822206a
“Sequential or superexchange mechanism in bridged electron transfer distinguished by dynamics at a bridging molecule”
Journal Pubmed - Keisuke Saito*, Hitoshi. Sumi
J. Chem. Phys. 131 (2009), 134101. doi: 10.1063/1.3223280
“Unifed expression for the rate constant of the bridged electron transfer derived by renormalization”
Journal Pubmed - Keisuke Saito*, Kazuhiro Yanagi, Richard J. Cogdell, and Hideki. Hashimoto*
J. Chem. Phys. 134 (2011), 044138. doi: DOI: 10.1063/1.3524339
“A comparison of the Liptay theory of electroabsorption spectroscopy with the sum-over-state model and its modification for the degenerate case”
Journal Pubmed - Keisuke Saito, Toyokazu Ishida, Miwa Sugiura, Keisuke Kawakami, Yasufumi Umena, Nobuo Kamiya, Jian-Ren Shen, and Hiroshi Ishikita*
J. Am. Chem. Soc. 133 (2011) 14379-1438. doi: 10.1021/ja203947k
“Distribution of the cationic state over the chlorophyll pair of photosystem II reaction center”
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Biophys. J. 101 (2011) 2018-2025. doi: 10.1016/j.bpj.2011.09.010
“Cationic state distribution over the P700 chlorophyll pair in Photosystem I”
Journal Pubmed - Keisuke Saito, Jian-Ren Shen, Toyokazu Ishida, and Hiroshi Ishikita*
Biochemistry 50 (2011) 9836-9844. doi: 10.1021/bi201366j
“Short hydrogen-bond between redox-active tyrosine YZ and D1-His190 in the photosystem II crystal structure”
Journal Pubmed - Misturu Sugisaki*, Daisuke Kosumi, Keisuke Saito, Richard J. Cogdell, Hideki Hashimoto,
Phys. Rev. B 85 (2012), 245408. doi: 10.1039/c5cp01388g
“Generation of coherently coupled vibronic oscillations in carotenoids”,
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 167-172. doi: 10.1073/pnas.1113599108 [Direct Submission, Edited by A. Fersht]
“Energetics of short hydrogen bonds in photoactive yellow protein”
Journal Pubmed - Keisuke Saito, Jian-Ren Shen, and Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics) 1817 (2012) 1191-1195. doi: 10.1016/j.bbabio.2011.12.003
“Cationic state distribution over the chlorophyll d-containing PD1/PD2 pair in photosystem II”
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Biochemistry 51 (2012) 1171-1177. doi: 10.1021/bi201877e
“H atom positions and nuclear magnetic resonance chemical shifts of short H bonds in photoactive yellow protein”
Journal Pubmed - Keisuke Saito, Jian-Ren Shen, and Hiroshi Ishikita*
Biophys. J. 102 (2012) 2634-2640. doi: 10.1016/j.bpj.2012.04.016
“Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from Thermosynechococcus vulcanus“
Journal Pubmed - Keisuke Saito, Yasufumi Umena, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya, and Hiroshi Ishikita*
Biochemistry 51 (2012) 4290-4299. doi: 10.1021/bi300428s
“Deformation of chlorin rings in the photosystem II crystal structure”
Journal Pubmed - Keisuke Saito, Hideki Kandori, and Hiroshi Ishikita*
J. Biol. Chem. 287 (2012) 34009-34018. doi: 10.1074/jbc.M112.388348
“Factors that differentiate the H-bond strengths of water near the Schiff bases in bacteriorhodopsin and Anabaena sensory rhodopsin”
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics) 827 (2013) 387-394. doi: 10.1016/j.bbabio.2012.11.009
“Formation of an unusually short hydrogen bond in photoactive yellow protein”
Journal Pubmed - Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 954-959. doi: 10.1073/pnas.1212957110 [Direct Submission, Edited by P. Joliot]
“Mechanism of proton-coupled quinone reduction in Photosystem II”
Journal Pubmed - Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 7690-7695. doi: 10.1073/pnas.1300817110 [Direct Submission, Edited by H. Gray]
“Mechanism of Tyrosine D oxidation in Photosystem II”
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics) 1837 (2014) 159-166. doi: 10.1016/j.bbabio.2013.09.013
“Influence of the Ca2+ ion on the Mn4Ca conformation and the H-bond network arrangement in Photosystem II”
Journal Pubmed - Hiroshi Ishikita* and Keisuke Saito
J. R. Soc. Interface 11(2014) 20130518 doi: 10.1098/rsif.2013.0518
“Proton transfer reactions and hydrogen-bond networks in protein environments”
Journal Pubmed - Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
Nat. Commun. 6 (2015) 8488. doi: 10.1038/ncomms9488
“Energetics of proton release on the first oxidation step in the water-oxidizing enzyme”
Journal Pubmed - Tomohiro Takaoka, Naoki Sakashita, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. Lett. 7 (2016) 1925-1932. doi: 10.1021/acs.jpclett.6b00656
“pKa of a proton conducting water chain in photosystem II”
Journal Pubmed - Keisuke Saito*, Naoki Sakashita, and Hiroshi Ishikita
Aust. J. Chem. 69 (2016) 991-998. doi:10.1071/CH16248
“Energetics of the proton transfer pathway for tyrosine D in photosystem II”
Journal - Ryo Hasegawa, Keisuke Saito, Tomohiro Takaoka, and Hiroshi Ishikita*
Photosynth. Res. 133 (2017) 297-304. doi: 10.1007/s11120-017-0382-y
“pKa of ubiquinone, menaquinone, phylloquinone, plastoquinone, and rhodoquinone in aqueous solution”
Journal Pubmed - Naoki Sakashita, Hiroshi C. Watanabe, Takuya Ikeda, Keisuke Saito, and Hiroshi Ishikita*
Biochemistry 56 (2017) 3049-3057. doi: 10.1021/acs.biochem.7b00220
“Origins of water molecules in the photosystem II crystal structure”
Journal Pubmed - Takuya Ikeda, Keisuke Saito, Ryo Hasegawa, and Hiroshi Ishikita*
Angew. Chem. 129 (2017) 9279-9282. doi: 10.1002/ange.201705512
Angew. Chem. Int. Ed. 56 (2017) 9151-9154. doi: 10.1002/anie.201705512
“Existence of isolated H3O+ in the protein interior”
Journal Pubmed - Shinnosuke Kishi, Keisuke Saito, Yuki Kato, and Hiroshi Ishikita*
Photosynth. Res. 134 (2017) 193-200. doi: 10.1007/s11120-017-0433-4
“Redox potentials of ubiquinone, menaquinone, phylloquinone, and plastoquinone in aqueous solution”
Journal Pubmed - Chiasa Uragamia, Keisuke Saito, Masayuki Yoshizawa, Péter Molnárd, and Hideki Hashimoto*
Arch. Biochem. Biophys. 650 (2018) 49-58 doi: 10.1016/j.abb.2018.04.021
”Unified analysis of optical absorption spectra of carotenoids based on a stochastic model”
Journal Pubmed - Keisuke Saito*, Takumi Suzuki, and Hiroshi Ishikita
J. Photochem. Photobiol. A 358 (2018) 422-431. doi: 10.1016/j.jphotochem.2017.10.003
“Absorption-energy calculations of chlorophyll a and b with an explicit solvent model”
Journal - Keisuke Kawashima, Tomohiro Takaoka, Hiroki Kimura, Keisuke Saito, and Hiroshi Ishikita*
Nat. Commun. 9 (2018) 1247. doi: 10.1038/s41467-018-03545-w
“O2 evolution and recovery of the water-oxidizing enzyme”
Journal Pubmed - Yoshitaka Saga*, Keiya Hirota, Sayaka Matsui, Hitoshi Asakawa, Hiroshi Ishikita, Keisuke Saito*
Biochemistry 57 (2018) 3075-3083. doi: 10.1021/acs.biochem.8b00259
“Selective removal of B800 bacteriochlorophyll a from the light-harvesting complex 2 of the purple photosynthetic bacterium Phaeospirillum molischianum”
Journal Pubmed - Keisuke Kawashima, Keisuke Saito, and Hiroshi Ishikita*
Biochemistry 57 (2018) 4997-5004. doi: 10.1021/acs.biochem.8b00574
“Mechanism of radical formation in the H-bond network of D1-Asn298 in photosystem II”
Journal Pubmed - Keisuke Saito and Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics)1860 (2019) 148059. doi: 10.1016/j.bbabio.2019.148059
“Mechanism of protonation of the over-reduced Mn4CaO5 cluster in photosystem II”
Journal Pubmed - Keiichi Kojima, Tetsuya Ueta, Tomoyasu Noji, Keisuke Saito, Kanae Kanehara, Susumu Yoshizawa, Hiroshi Ishikita, Yuki Sudo*
Sci. Rep. 10 (2020) 282. doi: 10.1038/s41598-019-57122-2
“Vectorial proton transport mechanism of RxR, a phylogenetically distinct and thermally stable microbial rhodopsin”
Journal Pubmed - Manoj Mandal, Keisuke Kawashima, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. Lett. 11 (2020) 249-255. doi: 10.1021/acs.jpclett.9b02831
“Redox potential of the oxygen-evolving complex in the electron transfer cascade of photosystem II”
Journal Pubmed - Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 6373-6382. doi: 10.1073/pnas.2000895117 [Direct Submission, Edited by P. Falkowski]
“Acquirement of water-splitting ability and alteration of charge-separation mechanism in photosynthetic reaction centers”
Journal Pubmed - Naoki Sakashita, Hiroshi Ishikita, and Keisuke Saito*
Phys. Chem. Chem. Phys. 22 (2020) 15831-15841. doi: 10.1039/d0cp00295
“Rigidly hydrogen-bonded water molecules facilitate proton transfer in photosystem II”
Journal Pubmed - Keisuke Saito, Minesato Nakagawa, and Hiroshi Ishikita*
Commun. Chem. 3 (2020) 89. doi: 10.1038/s42004-020-00336-7
“pKa of the ligand water molecules in the oxygen-evolving Mn4CaO5 cluster in photosystem II”
Journal - Keisuke Saito, Manoj Mandal, and Hiroshi Ishikita*
Biochemistry 59 (2020) 3216-3224. doi: 10.1021/acs.biochem.0c00177
“Energetics of ionized water molecules in the H-bond network near the Ca2+ and Cl– binding sites in photosystem II”
Journal Pubmed - Keisuke Saito*, Koji Mitsuhashi, and Hiroshi Ishikita*
J. Photochem. Photobiol. A 402 (2020) 112799. doi: 10.1016/j.jphotochem.2020.112799
“Dependence of the chlorophyll wavelength on the orientation of a charged group: why does the accessory chlorophyll have a low site energy in photosystem II?”
Journal - Keisuke Saito, Manoj Mandal, and Hiroshi Ishikita*
Phys. Chem. Chem. Phys. 22 (2020) 25467-25473. doi: 10.1039/D0CP04265J
“Redox potentials along the redox-active low-barrier H-bonds in electron transfer pathways”
Journal Pubmed - Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. Lett. 11 (2020) 10262-10268. doi: 10.1021/acs.jpclett.0c02868
“The nature of the short oxygen-oxygen distance in the Mn4CaO6 complex of the photosystem II crystals”
Journal Pubmed - Masaki Tsujimura, Tomoyasu Noji, Keisuke Saito, Keiichi Kojima, Yuki Sudo, and Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics) 862 (2021) 148349. doi: 10.1016/j.bbabio.2020.148349
“Mechanism of absorption wavelength shifts in anion channelrhodopsin-1 mutants”
Journal Pubmed - Hiroshi Kuroda1, Keisuke Kawashima1, Kazuyo Ueda, Takuya Ikeda, Keisuke Saito, Ryo Ninomiya, Chisato Hida, Yuichiro Takahashi*, Hiroshi Ishikita*
Biochim. Biophys. Acta (Bioenergetics) 1862 (2021) 148329. doi: 10.1016/j.bbabio.2020.148329
“Proton transfer pathway from the oxygen-evolving complex in photosystem II substantiated by extensive mutagenesis”
1These authors contributed equally to this work.
Journal Pubmed - Koji Mitsuhashi, Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. B 125 (2021) 2879-2885. doi: 10.1021/acs.jpcb.0c10885
“Nature of asymmetric electron transfer in the symmetric pathways of photosystem I”
Journal Pubmed - Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. Lett. 12 (2021) 4032-4037. doi: 10.1021/acs.jpclett.1c00814
“Two distinct oxygen-radical conformations in the X‑ray free electron laser structures of photosystem II”
Journal Pubmed - Hiroyuki Tamura*, Keisuke Saito, and Hiroshi Ishikita
Chem. Sci. 12 (2021) 8131-8140. doi: 10.1039/D1SC01497H
“The origin of unidirectional charge separation in photosynthetic reaction centers: nonadiabatic quantum dynamics of exciton and charge in the pigment-protein complexes”
Journal Pubmed - Keisuke Saito*, Minesato Nakagawa, Manoj Mandal, and Hiroshi Ishikita*
Photosynth. Res. 148 (2021) 153-159. doi: 10.1007/s11120-021-00846-y
“Role of redox-inactive metals in controlling the redox potential of heterometallic manganese-oxido clusters”
Journal Pubmed - Yu Sugo, Keisuke Saito, and Hiroshi Ishikita*
Proc. Natl. Acad. Sci. U. S. A. 118 (2021) e2103203118. doi: 10.1073/pnas.2103203118
“Mechanism of the formation of proton transfer pathways in photosynthetic reaction centers”
Journal Pubmed - Tomoki Kanda1, Keisuke Saito1, and Hiroshi Ishikita*
J. Phys. Chem. Lett. 12 (2021) 7431-7438. doi: 10.1021/acs.jpclett.1c01896
“Electron acceptor-donor Fe sites in the iron-sulfur cluster of photosynthetic electron-transfer pathways”
1These authors contributed equally to this work.
Journal Pubmed - Yasunori Saitoh1, Namiki Mitani-Ueno1, Keisuke Saito1, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, and Michihiro Suga *
Nat. Commun. 12 (2021) 6236. doi: 10.1038/s41467-021-26535-x
“Structural basis for high selectivity of a rice silicon channel Lsi1”
1These authors contributed equally to this work.
Journal Pubmed - Hiroyuki Tamura*, Keisuke Saito, and Hiroshi Ishikita
J. Phys. Chem. B 125 (2021) 13460-13466. doi: 10.1021/acs.jpcb.1c09538
“Long-range electron tunneling from the primary to secondary quinones in photosystem II enhanced by hydrogen bonds with non-heme Fe complex”
Journal Pubmed - Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. B 126 (2022) 123-131. doi: 10.1021/acs.jpcb.1c09176
“Requirement of chloride for downhill electron transfer pathway from the water-splitting center in natural photosynthesis”
Journal Pubmed - Yutaro Saito, Hiroyuki Yatabe, Iori Tamura, Yohei Kondo, Ryo Ishida, Tomohiro Seki, Keita Hiraga, Akihiro Eguchi, Yoichi Takakusagi, Keisuke Saito, Hiroshi Ishikita, Kazutoshi Yamamoto, Murali C. Krishna*, and Shinsuke Sando*
Sci. Adv. 8 (2022) eabj2667. doi: 10.1126/sciadv.abj2667
“Structure-guided design enables development of a hyperpolarized molecular probe for the detection of aminopeptidase N activity in vivo“
Journal Pubmed - Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Soc. Jpn. 91 (2022) 091012. doi: 10.7566/JPSJ.91.091012
“Release of electrons and protons from substrate water molecules at the oxygen-evolving complex in photosystem II”
Journal - Tomoki Kanda, Keisuke Saito, and Hiroshi Ishikita*
J. Phys. Chem. B 126 (2022) 3059-3066. doi: 10.1021/acs.jpcb.2c01320
“Mechanism of mixed-valence Fe2.5+…Fe2.5+ formation in Fe4S4 clusters in the ferredoxin binding motif”
Journal Pubmed - Masaki Tsujimura, Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
iScience 25 (2022) 104247. doi: 10.1016/j.isci.2022.104247
“Absorption wavelength along chromophore low-barrier hydrogen bonds”
Journal Pubmed - Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
ACS Phys. Chem Au (2022) doi: 10.1021/acsphyschemau.2c00019
“Release of the proton and formation of the low-barrier hydrogen bond between tyrosine D and D2-His189 in photosystem II”
Journal - Keisuke Saito*, Tianyang Xu, and Hiroshi Ishikita*
J. Phys. Chem. B 126 (2022) 4999-5006. doi: 10.1021/acs.jpcb.2c02193
“Correlation between C=O stretching vibrational frequency and pKa shift of carboxylic acids”
Journal Pubmed - Yu Sugo, Keisuke Saito, and Hiroshi Ishikita*
Biochemistry (2022) doi: 10.1021/acs.biochem.2c00324
“Conformational changes and H-bond rearrangements during quinone release in photosystem II”
Journal - Ko Imaizumi, Taishi Nishimura, Ryo Nagao, Keisuke Saito, Takeshi Nakano, Hiroshi Ishikita, Takumi Noguchi, and Kentaro Ifuku*
PNAS Nexus (2022) doi: 10.1093/pnasnexus/pgac136
“D139N mutation of PsbP enhances the oxygen-evolving activity of photosystem II through stabilized binding of a chloride ion”
Journal