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斉藤圭亮 (SAITO, Keisuke) CV

東京大学先端科学技術研究センター/大学院工学系研究科 応用化学専攻/石北研究室

准教授

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【若手研究者紹介:034】応用化学専攻 石北研究室 斉藤圭亮 准教授

略歴

2003年 3月筑波大学第三学群工学基礎学類卒業
2008年 3月筑波大学大学院数理物質科学研究科博士課程修了
2008年 4月大阪市立大学大学院理学研究科 博士研究員
2011年 1月京都大学生命科学系キャリアパス形成ユニット 特定研究員
2012年10月科学技術振興機構「藻類バイオエネルギー」領域 さきがけ研究者
2013年10月大阪大学大学院理学研究科 助教
2014年 6月東京大学大学院工学系研究科 助教
2014年10月東京大学先端科学技術研究センター 助教
2015年 5月東京大学先端科学技術研究センター 講師
2018年11月東京大学先端科学技術研究センター 准教授

PUBLICATION

  1. Keisuke Saito, Koishiro Mukai, and Hitoshi Sumi*,
    Chem. Phys. Lett. 401 (2005) 122-129. doi: 10.1016/j.cplett.2004.11.027
    “Excited states of pigments in photosystem II reaction centers of photosynthesis: characterization into a central dimer and remaining monomers”
    Journal
  2. Keisuke Saito, Tomoaki Kikuchi, Masaru Nakayama, Koishiro Mukai, and Hitoshi Sumi*
    J. Photochem. Photobiol. A 178 (2006) 271-270. doi: 10.1016/j.jphotochem.2005.10.038
    “A single chlorophyll in each of the core antennas CP43 and CP47 transferring excitation energies to the reaction center in photosystem II of photosynthesis”
    Journal 
  3. Keisuke Saito, Koishiro Mukai, and Hitoshi Sumi*
    Chem. Phys. 326 (2006) 221-229. doi: 10.1016/j.chemphys.2005.12.012
    “The importance of a hot-sequential mechanism in triplet-state formation by charge recombination in reaction centers of bacterial photosynthesis”
    Journal 
  4. Keisuke Saito*, Tomoaki Kikuchi, Koishiro Mukai, and Hitoshi. Sumi,
    Phys. Chem. Chem. Phys. 11 (2009) 5290-5301. doi: 10.1039/b822206a
    “Sequential or superexchange mechanism in bridged electron transfer distinguished by dynamics at a bridging molecule”
    Journal Pubmed
  5. Keisuke Saito*, Hitoshi. Sumi
    J. Chem. Phys. 131 (2009), 134101. doi: 10.1063/1.3223280
    “Unifed expression for the rate constant of the bridged electron transfer derived by renormalization”
    Journal Pubmed
  6. Keisuke Saito*, Kazuhiro Yanagi, Richard J. Cogdell, and Hideki. Hashimoto*
    J. Chem. Phys. 134 (2011), 044138. doi: DOI: 10.1063/1.3524339
    “A comparison of the Liptay theory of electroabsorption spectroscopy with the sum-over-state model and its modification for the degenerate case”
    Journal Pubmed
  7. Keisuke Saito, Toyokazu Ishida, Miwa Sugiura, Keisuke Kawakami, Yasufumi Umena, Nobuo Kamiya, Jian-Ren Shen, and Hiroshi Ishikita*
    J. Am. Chem. Soc. 133 (2011) 14379-1438. doi: 10.1021/ja203947k
    “Distribution of the cationic state over the chlorophyll pair of photosystem II reaction center”
    Journal Pubmed
  8. Keisuke Saito and Hiroshi Ishikita*
    Biophys. J. 101 (2011) 2018-2025. doi: 10.1016/j.bpj.2011.09.010
    “Cationic state distribution over the P700 chlorophyll pair in Photosystem I”
    Journal Pubmed
  9. Keisuke Saito, Jian-Ren Shen, Toyokazu Ishida, and Hiroshi Ishikita*
    Biochemistry 50 (2011) 9836-9844. doi: 10.1021/bi201366j
    “Short hydrogen-bond between redox-active tyrosine YZ and D1-His190 in the photosystem II crystal structure”
    Journal Pubmed
  10. Misturu Sugisaki*, Daisuke Kosumi, Keisuke Saito, Richard J. Cogdell, Hideki Hashimoto,
    Phys. Rev. B 85 (2012), 245408. doi: 10.1039/c5cp01388g
    “Generation of coherently coupled vibronic oscillations in carotenoids”,
    Journal Pubmed
  11. Keisuke Saito and Hiroshi Ishikita*
    Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 167-172. doi: 10.1073/pnas.1113599108 [Direct Submission, Edited by A. Fersht]
    “Energetics of short hydrogen bonds in photoactive yellow protein”
    Journal Pubmed
  12. Keisuke Saito, Jian-Ren Shen, and Hiroshi Ishikita*
    Biochim. Biophys. Acta  (Bioenergetics) 1817 (2012) 1191-1195. doi: 10.1016/j.bbabio.2011.12.003
    “Cationic state distribution over the chlorophyll d-containing PD1/PD2 pair in photosystem II”
    Journal Pubmed
  13. Keisuke Saito and Hiroshi Ishikita*
    Biochemistry 51 (2012) 1171-1177. doi: 10.1021/bi201877e
    “H atom positions and nuclear magnetic resonance chemical shifts of short H bonds in photoactive yellow protein”
    Journal Pubmed
  14. Keisuke Saito, Jian-Ren Shen, and Hiroshi Ishikita*
    Biophys. J. 102 (2012) 2634-2640. doi: 10.1016/j.bpj.2012.04.016
    “Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from Thermosynechococcus vulcanus
    Journal Pubmed
  15. Keisuke Saito, Yasufumi Umena, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya, and Hiroshi Ishikita*
    Biochemistry 51 (2012) 4290-4299. doi: 10.1021/bi300428s
    “Deformation of chlorin rings in the photosystem II crystal structure”
    Journal Pubmed
  16. Keisuke Saito, Hideki Kandori, and Hiroshi Ishikita*
    J. Biol. Chem. 287 (2012) 34009-34018. doi: 10.1074/jbc.M112.388348
    “Factors that differentiate the H-bond strengths of water near the Schiff bases in bacteriorhodopsin and Anabaena sensory rhodopsin”
    Journal Pubmed
  17. Keisuke Saito and Hiroshi Ishikita*
    Biochim. Biophys. Acta (Bioenergetics) 827 (2013) 387-394. doi: 10.1016/j.bbabio.2012.11.009
    “Formation of an unusually short hydrogen bond in photoactive yellow protein”
    Journal Pubmed
  18. Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
    Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 954-959. doi: 10.1073/pnas.1212957110 [Direct Submission, Edited by P. Joliot]
    “Mechanism of proton-coupled quinone reduction in Photosystem II”
    Journal Pubmed
  19. Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
    Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 7690-7695. doi: 10.1073/pnas.1300817110 [Direct Submission, Edited by H. Gray]
    “Mechanism of Tyrosine D oxidation in Photosystem II”
    Journal Pubmed
  20. Keisuke Saito and Hiroshi Ishikita*
    Biochim. Biophys. Acta  (Bioenergetics) 1837 (2014) 159-166. doi: 10.1016/j.bbabio.2013.09.013
    “Influence of the Ca2+ ion on the Mn4Ca conformation and the H-bond network arrangement in Photosystem II”
    Journal Pubmed
  21. Hiroshi Ishikita* and Keisuke Saito
    J. R. Soc. Interface 11(2014) 20130518 doi: 10.1098/rsif.2013.0518
    “Proton transfer reactions and hydrogen-bond networks in protein environments”
    Journal Pubmed
  22. Keisuke Saito, A. William Rutherford, and Hiroshi Ishikita*
    Nat. Commun. 6 (2015) 8488. doi: 10.1038/ncomms9488
    “Energetics of proton release on the first oxidation step in the water-oxidizing enzyme”
    Journal Pubmed
  23. Tomohiro TakaokaNaoki SakashitaKeisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. Lett. (2016) 1925-1932. doi: 10.1021/acs.jpclett.6b00656
    “pKa of a proton conducting water chain in photosystem II”
    Journal Pubmed
  24. Keisuke Saito*Naoki Sakashita, and Hiroshi Ishikita
    Aust. J. Chem. 69 (2016) 991-998. doi:10.1071/CH16248
    “Energetics of the proton transfer pathway for tyrosine D in photosystem II”
    Journal
  25. Ryo Hasegawa, Keisuke Saito, Tomohiro Takaoka, and Hiroshi Ishikita*
    Photosynth. Res. 133 (2017) 297-304. doi: 10.1007/s11120-017-0382-y
    “pKa of ubiquinone, menaquinone, phylloquinone, plastoquinone, and rhodoquinone in aqueous solution”
    Journal Pubmed
  26. Naoki Sakashita, Hiroshi C. Watanabe, Takuya Ikeda, Keisuke Saito, and Hiroshi Ishikita*
    Biochemistry 56 (2017) 3049-3057. doi: 10.1021/acs.biochem.7b00220
    “Origins of water molecules in the photosystem II crystal structure”
    Journal Pubmed
  27. Takuya IkedaKeisuke SaitoRyo Hasegawa, and Hiroshi Ishikita*
    Angew. Chem. 129 (2017) 9279-9282. doi: 10.1002/ange.201705512
    Angew. Chem. Int. Ed. 56
     (2017) 9151-9154. doi: 10.1002/anie.201705512
    “Existence of isolated H3O+ in the protein interior”
    Journal Pubmed
  28. Shinnosuke KishiKeisuke Saito, Yuki Kato, and Hiroshi Ishikita*
    Photosynth. Res. 134 (2017) 193-200. doi: 10.1007/s11120-017-0433-4
    “Redox potentials of ubiquinone, menaquinone, phylloquinone, and plastoquinone in aqueous solution”
    Journal Pubmed
  29. Chiasa Uragamia, Keisuke Saito, Masayuki Yoshizawa, Péter Molnárd, and Hideki Hashimoto*
    Arch. Biochem. Biophys. 650 (2018) 49-58 doi: 10.1016/j.abb.2018.04.021
    ”Unified analysis of optical absorption spectra of carotenoids based on a stochastic model”
    Journal Pubmed
  30. Keisuke Saito*Takumi Suzuki, and Hiroshi Ishikita
    J. Photochem. Photobiol. A 358 (2018) 422-431. doi: 10.1016/j.jphotochem.2017.10.003
    “Absorption-energy calculations of chlorophyll a and b with an explicit solvent model”
    Journal
  31. Keisuke Kawashima, Tomohiro Takaoka, Hiroki Kimura, Keisuke Saito, and Hiroshi Ishikita*
    Nat. Commun. 9 (2018) 1247. doi: 10.1038/s41467-018-03545-w
    “O2 evolution and recovery of the water-oxidizing enzyme”
    Journal Pubmed
  32. Yoshitaka Saga*, Keiya Hirota, Sayaka Matsui, Hitoshi Asakawa, Hiroshi IshikitaKeisuke Saito*
    Biochemistry 57 (2018) 3075-3083. doi: 10.1021/acs.biochem.8b00259
    “Selective removal of B800 bacteriochlorophyll a from the light-harvesting complex 2 of the purple photosynthetic bacterium Phaeospirillum molischianum
    Journal Pubmed
  33. Keisuke KawashimaKeisuke Saito, and Hiroshi Ishikita*
    Biochemistry 57 (2018) 4997-5004. doi: 10.1021/acs.biochem.8b00574
    “Mechanism of radical formation in the H-bond network of D1-Asn298 in photosystem II”
    Journal Pubmed
  34. Keisuke Saito and Hiroshi Ishikita*
    Biochim. Biophys. Acta (Bioenergetics)1860 (2019) 148059. doi: 10.1016/j.bbabio.2019.148059
    “Mechanism of protonation of the over-reduced Mn4CaO5 cluster in photosystem II”
    Journal Pubmed
  35. Keiichi Kojima, Tetsuya Ueta, Tomoyasu NojiKeisuke Saito, Kanae Kanehara, Susumu Yoshizawa, Hiroshi Ishikita, Yuki Sudo*
    Sci. Rep. 10 (2020) 282. doi: 10.1038/s41598-019-57122-2
    “Vectorial proton transport mechanism of RxR, a phylogenetically distinct and thermally stable microbial rhodopsin”
    Journal Pubmed
  36. Manoj Mandal, Keisuke Kawashima, Keisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. Lett. 11 (2020) 249-255. doi: 10.1021/acs.jpclett.9b02831
    “Redox potential of the oxygen-evolving complex in the electron transfer cascade of photosystem II”
    Journal Pubmed
  37. Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
    Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 6373-6382. doi: 10.1073/pnas.2000895117 [Direct Submission, Edited by P. Falkowski]
    “Acquirement of water-splitting ability and alteration of charge-separation mechanism in photosynthetic reaction centers”
    Journal Pubmed
  38. Naoki SakashitaHiroshi Ishikita, and Keisuke Saito*
    Phys. Chem. Chem. Phys. 22 (2020) 15831-15841. doi: 10.1039/d0cp00295
    “Rigidly hydrogen-bonded water molecules facilitate proton transfer in photosystem II”
    Journal Pubmed
  39. Keisuke SaitoMinesato Nakagawa, and Hiroshi Ishikita*
    Commun. Chem. 3 (2020) 89. doi: 10.1038/s42004-020-00336-7
    “pKa of the ligand water molecules in the oxygen-evolving Mn4CaO5 cluster in photosystem II”
    Journal
  40. Keisuke SaitoManoj Mandal, and Hiroshi Ishikita*
    Biochemistry 59 (2020) 3216-3224. doi: 10.1021/acs.biochem.0c00177
    “Energetics of ionized water molecules in the H-bond network near the Ca2+ and Cl binding sites in photosystem II”
    Journal Pubmed
  41. Keisuke Saito*Koji Mitsuhashi, and Hiroshi Ishikita*
    J. Photochem. Photobiol. A 402 (2020) 112799. doi: 10.1016/j.jphotochem.2020.112799
    “Dependence of the chlorophyll wavelength on the orientation of a charged group: why does the accessory chlorophyll have a low site energy in photosystem II?”
    Journal
  42. Keisuke SaitoManoj Mandal, and Hiroshi Ishikita*
    Phys. Chem. Chem. Phys. 22 (2020) 25467-25473. doi: 10.1039/D0CP04265J
    “Redox potentials along the redox-active low-barrier H-bonds in electron transfer pathways”
    Journal Pubmed
  43. Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. Lett. 11 (2020) 10262-10268. doi: 10.1021/acs.jpclett.0c02868
    “The nature of the short oxygen-oxygen distance in the Mn4CaO6 complex of the photosystem II crystals”
    Journal Pubmed
  44. Masaki TsujimuraTomoyasu NojiKeisuke Saito, Keiichi Kojima, Yuki Sudo, and Hiroshi Ishikita*
    Biochim. Biophys. Acta (Bioenergetics) 862 (2021) 148349. doi: 10.1016/j.bbabio.2020.148349
    “Mechanism of absorption wavelength shifts in anion channelrhodopsin-1 mutants”
    Journal Pubmed
  45. Hiroshi Kuroda1Keisuke Kawashima1, Kazuyo Ueda, Takuya IkedaKeisuke Saito, Ryo Ninomiya, Chisato Hida, Yuichiro Takahashi*, Hiroshi Ishikita*
    Biochim. Biophys. Acta (Bioenergetics) 1862 (2021) 148329. doi: 10.1016/j.bbabio.2020.148329
    “Proton transfer pathway from the oxygen-evolving complex in photosystem II substantiated by extensive mutagenesis”
    1These authors contributed equally to this work.
    Journal Pubmed
  46. Koji Mitsuhashi, Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. B 125 (2021) 2879-2885. doi: 10.1021/acs.jpcb.0c10885
    “Nature of asymmetric electron transfer in the symmetric pathways of photosystem I”
    Journal Pubmed
  47. Manoj MandalKeisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. Lett. 12 (2021) 4032-4037. doi: 10.1021/acs.jpclett.1c00814
    “Two distinct oxygen-radical conformations in the X‑ray free electron laser structures of photosystem II”
    Journal Pubmed
  48. Hiroyuki Tamura*, Keisuke Saito, and Hiroshi Ishikita
    Chem. Sci. 12 (2021) 8131-8140. doi: 10.1039/D1SC01497H
    “The origin of unidirectional charge separation in photosynthetic reaction centers: nonadiabatic quantum dynamics of exciton and charge in the pigment-protein complexes”
    Journal Pubmed
  49. Keisuke Saito*, Minesato Nakagawa, Manoj Mandal, and Hiroshi Ishikita*
    Photosynth. Res. 148 (2021) 153-159. doi: 10.1007/s11120-021-00846-y
    “Role of redox-inactive metals in controlling the redox potential of heterometallic manganese-oxido clusters”
    Journal Pubmed
  50. Yu Sugo, Keisuke Saito, and Hiroshi Ishikita*
    Proc. Natl. Acad. Sci. U. S. A. 118 (2021) e2103203118. doi: 10.1073/pnas.2103203118
    “Mechanism of the formation of proton transfer pathways in photosynthetic reaction centers”
    Journal Pubmed
  51. Tomoki Kanda1Keisuke Saito1, and Hiroshi Ishikita*
    J. Phys. Chem. Lett. 12 (2021) 7431-7438. doi: 10.1021/acs.jpclett.1c01896
    “Electron acceptor-donor Fe sites in the iron-sulfur cluster of photosynthetic electron-transfer pathways”
    1These authors contributed equally to this work.
    Journal Pubmed
  52. Yasunori Saitoh1, Namiki Mitani-Ueno1, Keisuke Saito1, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, and Michihiro Suga *
    Nat. Commun. 12 (2021) 6236. doi: 10.1038/s41467-021-26535-x
    “Structural basis for high selectivity of a rice silicon channel Lsi1”
    1These authors contributed equally to this work.
    Journal Pubmed
  53. Hiroyuki Tamura*, Keisuke Saito, and Hiroshi Ishikita
    J. Phys. Chem. B 125 (2021) 13460-13466. doi: 10.1021/acs.jpcb.1c09538
    “Long-range electron tunneling from the primary to secondary quinones in photosystem II enhanced by hydrogen bonds with non-heme Fe complex”
    Journal Pubmed
  54. Manoj MandalKeisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. B 126 (2022) 123-131. doi: 10.1021/acs.jpcb.1c09176
    “Requirement of chloride for downhill electron transfer pathway from the water-splitting center in natural photosynthesis”
    Journal Pubmed
  55. Yutaro Saito, Hiroyuki Yatabe, Iori Tamura, Yohei Kondo, Ryo Ishida, Tomohiro Seki, Keita Hiraga, Akihiro Eguchi, Yoichi Takakusagi, Keisuke Saito, Hiroshi Ishikita, Kazutoshi Yamamoto, Murali C. Krishna*, and Shinsuke Sando*
    Sci. Adv. 8 (2022) eabj2667. doi: 10.1126/sciadv.abj2667
    “Structure-guided design enables development of a hyperpolarized molecular probe for the detection of aminopeptidase N activity in vivo
    Journal Pubmed
  56. Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
    J. Phys. Soc. Jpn. 91 (2022) 091012. doi: 10.7566/JPSJ.91.091012
    “Release of electrons and protons from substrate water molecules at the oxygen-evolving complex in photosystem II”
    Journal
  57. Tomoki Kanda, Keisuke Saito, and Hiroshi Ishikita*
    J. Phys. Chem. B 126 (2022) 3059-3066. doi: 10.1021/acs.jpcb.2c01320
    “Mechanism of mixed-valence Fe2.5+…Fe2.5+ formation in Fe4S4 clusters in the ferredoxin binding motif”
    Journal Pubmed
  58. Masaki Tsujimura, Hiroyuki Tamura, Keisuke Saito, and Hiroshi Ishikita*
    iScience 25 (2022) 104247. doi: 10.1016/j.isci.2022.104247
    “Absorption wavelength along chromophore low-barrier hydrogen bonds”
    Journal Pubmed
  59. Manoj Mandal, Keisuke Saito, and Hiroshi Ishikita*
    ACS Phys. Chem Au (2022) doi: 10.1021/acsphyschemau.2c00019
    “Release of the proton and formation of the low-barrier hydrogen bond between tyrosine D and D2-His189 in photosystem II”
    Journal
  60. Keisuke Saito*, Tianyang Xu, and Hiroshi Ishikita*
    J. Phys. Chem. B 126 (2022) 4999-5006. doi: 10.1021/acs.jpcb.2c02193
    “Correlation between C=O stretching vibrational frequency and pKa shift of carboxylic acids”
    Journal Pubmed
  61. Yu Sugo, Keisuke Saito, and Hiroshi Ishikita*
    Biochemistry (2022) doi: 10.1021/acs.biochem.2c00324
    “Conformational changes and H-bond rearrangements during quinone release in photosystem II”
    Journal
  62. Ko Imaizumi, Taishi Nishimura, Ryo Nagao, Keisuke Saito, Takeshi Nakano, Hiroshi Ishikita, Takumi Noguchi, and Kentaro Ifuku*
    PNAS Nexus (2022) doi: 10.1093/pnasnexus/pgac136
    “D139N mutation of PsbP enhances the oxygen-evolving activity of photosystem II through stabilized binding of a chloride ion”
    Journal